spp1 rabbit antibody  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: The Old Genetically Heterogeneous Mouse Model Recapitulates Chronic and Persistent Idiopathic Pulmonary Fibrosis with Strong Senescence Signatures

doi: 10.1101/2025.07.22.665978

Figure Lengend Snippet: (A) Senescent genes identified among the DEGs at D24 from the SEN MAYO panel. (A-i) Leading edge genes identified in the SEN MAYO panel at D72. (B) Venn diagram indicating an overlap of DEGs between C57BL/6J and HET3 mice using the SEN MAYO senescence gene set. 11 genes from the SEN MAYO panel were found to be present in both sets. C57BL/6J data re-analyzed from . (B-i) Venn diagram comparing genes from Bleomycin-treated mice at D24 and D72 (3.5 mg/kg) with SEN MAYO senescence gene panel and Human IPF data. Data was re-analyzed from . (C, C-i) Gene expression of Spp1, Igf1 and Il6 of sham and bleomycin-treated mice. Rpl13a was used as housekeeping control. Significance calculated using unpaired t-test; data represented as mean ± SEM at D24 and D72. (D) Representative IHC images at D24 and D72 of sham and bleomycin-treated mice stained for SPP1. Scale bar = 50 um. (D-i) Spp1 positive cell quantification at D24 and (D-ii) D72. Data represented as mean of four different fields of view per mouse ± SEM, unpaired t-test, *p ≤ 0.05, ** p ≤ 0.002, ns ≥ 0.05. (E) Validation of Spp1 expression data via spatial transcriptomic analysis in mouse IPF samples. Mouse samples with IPF showcase higher expression of Spp1. (E-i) Validation of SPP1 expression in human IPF samples. Data was re-analyzed from Franzen et al., 2024 for both human and mouse tissues. IPF = Freshly frozen tissue. IPF lung tissue collected with severe tissue remodeling. Control = Healthy lung tissue no known evidence of lung disease.

Article Snippet: Rabbit anti-Spp1 primary antibody (Proteintech, cat no. 22952-1-AP) was used at a 1:300 dilution overnight at 4°C.

Techniques: Gene Expression, Control, Staining, Biomarker Discovery, Expressing

Journal: European journal of medical research

Article Title: Identification of diagnostic biomarkers and immune cell infiltration in tongue squamous cell carcinoma using bioinformatic approaches.

doi: 10.1186/s40001-024-01998-y

Figure Lengend Snippet: Fig. 5 SPP1 was highly expressed in tongue from patients with TSCC. (A) Immunohistochemical staining of SPP1 in cancerous and paracancer tissues of the tongue. (B) Representative immunofluorescence images of SPP1 (red) and CD68 (green) from cancerous and paracancer tissues of the tongue (Scale bars, 20 μm)

Article Snippet: The tissues were incubated overnight at room temperature with a rabbit anti-SPP1 antibody (1:50, Proteintech, 22952-1-AP).

Techniques: Immunohistochemical staining, Staining, Immunofluorescence

Journal: Cell Reports Medicine

Article Title: Monitoring melanoma patients on treatment reveals a distinct macrophage population driving targeted therapy resistance

doi: 10.1016/j.xcrm.2024.101611

Figure Lengend Snippet:

Article Snippet: Rabbit anti-human,mouse SPP1 antibody , ThermoFisher Scientific , Cat# PA5-141129; RRID:AB_2932581.

Techniques: Blocking Assay, Recombinant, Avidin-Biotin Assay, Plasmid Preparation, SYBR Green Assay, RNAscope, Selection, Transfection, Reverse Transcription, Flow Cytometry, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Negative Control, Software

Journal: Cells

Article Title: Dental Pulp Cell Transplantation Combined with Regenerative Endodontic Procedures Promotes Dentin Matrix Formation in Mature Mouse Molars

doi: 10.3390/cells13040348

Figure Lengend Snippet: Detection of odontoblast-like cells in the regenerated dental pulp-like tissue. Representative images of hematoxylin and eosin (H&E)-stained sections and immunohistochemistry (IHC) sections stained with nestin, osteopontin (OPN), and green fluorescent protein (GFP) antibodies, as well as osteocalcin. Nestin (odontoblast marker) is not detected in regenerative endodontic procedure (REP) groups, whereas in the REP + dental pulp cell (DPC) groups, the regenerated tissue contains nestin-stained cells that co-express GFP (arrows). OPN and osteocalcin were detected in both groups, with the former being more abundant in the cellular matrix of the REP + DPC group (n = 6 per group). Scale bars = 50 µm (arrows indicate nestin and GFP co-expressing cells). 4′,6-diamidino-2-phenylindole: DAPI, Osteopontin: OPN.

Article Snippet: For immunohistochemical (IHC) staining, the sections were deparaffinized, and heat-mediated antigen retrieval was performed in a water bath at 95–100 °C for 20 min with sodium citrate buffer (pH 6.0) using chicken anti-nestin antibody (NB100-1604, Novus Biologicals, Centennial, CO, USA) and osteopontin (OPN) antibody (88742, Cell Signaling Technology, Danvers, MA, USA) and Tris-EDTA (pH 9.0) using rabbit anti-CD31 antibody (28083-1-AP, Proteintech, Rosemont, IL, USA).

Techniques: Staining, Immunohistochemistry, Marker, Expressing